Deposition

Cryo-electron tomography of HeLa cells under mitochondrial proteostatic stress

Deposition ID:CZCDP-10344

Release Date: 2026-03-24

Last Modified: 2026-03-24

key visualization for Cryo-electron tomography of HeLa cells under mitochondrial proteostatic stress

Photo Caption: Central slab of 4 representative tomograms with selected sets of annotations.

Deposition Overview

In situ cryo-electron tomography data of FIB-milled HeLa PINK1-GFP cells under G-TPP-induced mitochondrial proteostatic stress and untreated control conditions. Includes raw tilt series movie frames, assembled tilt series, and WBP tomographic reconstructions from AreTomo3, and automated semantic segmentations (membrain-seg for membranes). Associated with the study of structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress.

Authors

Deposition Data

Annotations:412

Tomograms:824

Publications

DOI:10.1101/2025.10.06.680693

Related Databases

EMPIAR ID:EMPIAR-13145

Methods Summary

Annotations	Method Type	Method Details	Method Links
412	Automated	Prediction using membrain-seg	Source Code:membrain-seg +1 more

Deposited Data

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Data Contents

Tilt SeriesAvailable

FramesAvailable

CTFAvailable

AlignmentAvailable

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412 of 412 Annotations

Annotation Name	Object Shape Type	Method Type	Deposited In
100membrane Annotation ID: AN-133850	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P9_ts_007
100membrane Annotation ID: AN-133849	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P9_ts_006
100membrane Annotation ID: AN-133848	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P9_ts_005
100membrane Annotation ID: AN-133847	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P9_ts_004
100membrane Annotation ID: AN-133846	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P9_ts_003
100membrane Annotation ID: AN-133845	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P9_ts_002
100membrane Annotation ID: AN-133844	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P8_ts_008
100membrane Annotation ID: AN-133843	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P8_ts_007
100membrane Annotation ID: AN-133842	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P8_ts_006
100membrane Annotation ID: AN-133841	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P8_ts_005
100membrane Annotation ID: AN-133840	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P8_ts_004
100membrane Annotation ID: AN-133839	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P8_ts_003
100membrane Annotation ID: AN-133838	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P8_ts_002
100membrane Annotation ID: AN-133837	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P7_ts_008
100membrane Annotation ID: AN-133836	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P7_ts_007
100membrane Annotation ID: AN-133835	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P7_ts_006
100membrane Annotation ID: AN-133834	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P7_ts_005
100membrane Annotation ID: AN-133833	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P7_ts_004
100membrane Annotation ID: AN-133832	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P7_ts_003
100membrane Annotation ID: AN-133831	SegmentationMask	Automated	Dataset: Cryo-electron tomography of untreated HeLa cells (control)Run: 20230404_P7_ts_002