Deposition
CryoET of near-minimal cells Mycoplasma mycoides JCVI-Syn3A for the development of subtomogram averaging pipelines
- Deposition ID:CZCDP-10312
Release Date: 2024-10-15
Last Modified: 2024-10-15
Deposition Overview
Raw movie stacks, tilt series, alignments and cryo-eletron tomograms of synthetically reduced genome cells. JCVI-Syn3A has 493 genes (about half of the genes of the WT Mycoplasma Mycoides Capri GM12). Samples were prepared by plunge-freezing via Leica GP2. Contained data was collected on a Krios G4 using a Falcon 4i detector and SelctrisX energy filter. Tomograms were reconstructed using AreTomo3 v1.0.23. The deposition also includes locations of cytosolic ribosomes identified using slabpick and membrane segmenations created using membrain-seg.
Authors
Mallak Ali, Ariana Peck, Yue Yu, Jonathan Schwartz, John Glass, Shawn Zheng, Mohammadreza Paraan
Annotation Methods Summary
Datasets with Deposition Data
1 of 1 Datasets
Dataset Name | Organism | Runs With deposition data | Annotations Deposition only | Annotated Objects Deposition only | |
|---|---|---|---|---|---|
 | Dataset ID: DS-10442 Mallak Ali, Ariana Peck, Yue Yu, Jonathan Schwartz, ... , Mohammadreza Paraan | Mycoplasma mycoides subsp. capri str. GM12 | 262 | 523 |
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