Dataset

CryoET of isolated synaptic vesicles from mouse brain

Dataset ID:DS-10453

Release Date: 2025-11-17

Last Modified: 2025-11-17

key visualization for CryoET of isolated synaptic vesicles from mouse brain

Photo Caption: Central slab from a representative tomogram of a Mus musculus sample with selected set of annotations.

Dataset Overview

CryoET data of synaptic vesicles (SVs) isolated from mouse brain. Includes raw tiltseries movies, alignment, defocusm and tilt angle metadata, and reconstructed tomograms capturing vesicle ultrastructure and surrounding molecular features. Samples were plunge-frozen and imaged on a Titan Krios microscope equipped with a Falcon 4 detector and energy filter. Data processing was performed using TomoBEAR for workflow management. Aligned frames were motion-corrected with MotionCor2, and tiltseries alignment was carried out using DynamoTSA with manual refinement in IMOD based on 10-nm or 5-nm gold fiducial markers. Defocus values were estimated using Gctf, and CTF correction was applied with ctfphaseflip from IMOD. Final reconstructions were generated by weighted backprojection in IMOD.

Runs

95 of 95 Runs

Run Name	Tilt Series Quality Score	Objects
tomogram_003 Run ID: RN-18802	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_004 Run ID: RN-18803	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_005 Run ID: RN-18804	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_006 Run ID: RN-18805	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_007 Run ID: RN-18806	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_008 Run ID: RN-18807	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_009 Run ID: RN-18808	3 - Moderate	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_010 Run ID: RN-18809	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_011 Run ID: RN-18810	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_013 Run ID: RN-18811	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_014 Run ID: RN-18812	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_015 Run ID: RN-18813	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_016 Run ID: RN-18814	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_017 Run ID: RN-18815	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_018 Run ID: RN-18816	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_019 Run ID: RN-18817	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_020 Run ID: RN-18818	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_021 Run ID: RN-18819	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_022 Run ID: RN-18820	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram
tomogram_024 Run ID: RN-18821	5 - Excellent	clathrin coat GT clathrin complex GT proton-transporting V-type ATPase complex GT proton-transporting V-type ATPase, V0 domain GT	View Tomogram