Dataset
Cryo-electron tomography of purified E. coli 70S ribosomes acquired by fast-incremental single-exposure (FISE) tilt series
- Dataset ID:DS-10497
Release Date: 2026-07-09
Last Modified: 2026-07-09
Photo Caption: Central slab from a representative tomogram of the in vitro sample with selected set of annotations.
Dataset Overview
Purified 70S ribosomes from Escherichia coli strain DH5-alpha were imaged by cryo-electron tomography using the fast-incremental single-exposure (FISE) tilt-series acquisition method. Ribosomes were isolated from a cleared cell lysate by sucrose-cushion ultracentrifugation, mixed with 10 nm colloidal-gold fiducials, and plunge-frozen on Quantifoil grids. Twelve dose-symmetric FISE tilt series (-60 to +60 degrees, 3 degree increments, 2.1 Angstrom per pixel) were collected on a Titan Krios equipped with a Gatan K3 detector and energy filter, and subtomogram averaging of the ribosome particles yields a sub-nanometre-resolution map of the 70S ribosome. The raw tilt series were originally deposited as EMPIAR-10304; the tomograms in this dataset were reconstructed from those tilt series by the CryoET Data Portal team.
Publications
Related Databases
EMPIAR ID:EMPIAR-10304
EMDB ID:EMD-10211
Runs
12 of 12 Runs
Run Name | Tilt Series Quality Score | Objects | ||
|---|---|---|---|---|
 | tilt1 Run ID: RN-35416 | 5 - Excellent | -- | |
 | tilt10 Run ID: RN-35417 | 5 - Excellent | -- | |
 | tilt11 Run ID: RN-35418 | 5 - Excellent | -- | |
 | tilt12 Run ID: RN-35419 | 5 - Excellent | -- | |
 | tilt2 Run ID: RN-35420 | 5 - Excellent | -- | |
 | tilt3 Run ID: RN-35421 | 5 - Excellent | -- | |
 | tilt4 Run ID: RN-35422 | 5 - Excellent | -- | |
 | tilt5 Run ID: RN-35423 | 5 - Excellent | -- | |
 | tilt6 Run ID: RN-35424 | 5 - Excellent | -- | |
 | tilt7 Run ID: RN-35425 | 5 - Excellent | -- | |
 | tilt8 Run ID: RN-35426 | 5 - Excellent | -- | |
 | tilt9 Run ID: RN-35427 | 5 - Excellent | -- |