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Dataset

PP7 virus-like particles in E. coli at 3.0 Å (plunge-frozen)

  • Dataset ID:DS-10493

Release Date: 2026-05-13

Last Modified: 2026-05-13

key visualization for PP7 virus-like particles in E. coli at 3.0 Å (plunge-frozen)

Photo Caption: Central slab from a representative tomogram of a Escherichia coli BL21(DE3) sample with selected set of annotations.

Dataset Overview

Leviviridae PP7 VLP were inoculated into BL21(DE3) E. coli and plunge-frozen at the 1-hour timepoint. The targets for cryoET were selected based on high crowdedness and variable thickness (100-300 nm). The processing pipeline includes AreTomo3, Embrella, copick, octopi, RELION, py2rely, and pytom-match-pick. The most abundant geometry- the icosahedral T=3- was refined to 3.0 Å. Native E. coli protein complexes within the same tomograms (GroEL) were refined to 6.8 Å.

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Runs

402 of 402 Runs

Run Name
Tilt Series Quality Score
Objects
key visualization for 25oct20a_Position_1
25oct20a_Position_1

Run ID: RN-34482

5 - Excellent
  • Capsid protein
key visualization for 25oct20a_Position_10
25oct20a_Position_10

Run ID: RN-34483

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_100
25oct20a_Position_100

Run ID: RN-34484

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_100_2
25oct20a_Position_100_2

Run ID: RN-34485

5 - Excellent
  • Capsid protein
key visualization for 25oct20a_Position_100_3
25oct20a_Position_100_3

Run ID: RN-34486

5 - Excellent
  • Capsid protein
key visualization for 25oct20a_Position_100_4
25oct20a_Position_100_4

Run ID: RN-34487

5 - Excellent
  • Capsid protein
key visualization for 25oct20a_Position_101
25oct20a_Position_101

Run ID: RN-34488

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_101_2
25oct20a_Position_101_2

Run ID: RN-34489

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_102
25oct20a_Position_102

Run ID: RN-34490

3 - Moderate
  • Capsid protein
key visualization for 25oct20a_Position_10_2
25oct20a_Position_10_2

Run ID: RN-34502

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_103
25oct20a_Position_103

Run ID: RN-34491

5 - Excellent
  • Capsid protein
key visualization for 25oct20a_Position_104
25oct20a_Position_104

Run ID: RN-34492

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_104_2
25oct20a_Position_104_2

Run ID: RN-34493

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_105
25oct20a_Position_105

Run ID: RN-34494

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_106
25oct20a_Position_106

Run ID: RN-34495

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_106_2
25oct20a_Position_106_2

Run ID: RN-34496

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_107
25oct20a_Position_107

Run ID: RN-34497

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_108
25oct20a_Position_108

Run ID: RN-34498

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_108_2
25oct20a_Position_108_2

Run ID: RN-34499

4 - Good
  • Capsid protein
  • Chaperonin GroEL
    GT
key visualization for 25oct20a_Position_109
25oct20a_Position_109

Run ID: RN-34500

5 - Excellent
  • Capsid protein
  • Chaperonin GroEL
    GT
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