CEMOVIS of plant-type cell walls in Arabidopsis thaliana cultured cells

Cryo-electron tomograms of plant-type cell walls in liquid-cultured Arabidopsis thaliana cells. Cells were high-pressure frozen and processed by cryo-ultramicrotomy to produce vitreous cryosections (CEMOVIS / CETOVIS), capturing the cell wall between adjacent cells in a near-native state. Tilt series were collected on a 300 kV FEI Polara with a Gatan K2 SUMMIT camera using SerialEM in a dose-symmetric scheme; tomograms were reconstructed in IMOD using weighted back-projection with a SIRT-like filter applied during reconstruction.