Cryo-electron tomography of centrosomes in C. elegans embryonic cells

Cryo-electron tomography data of centrosomes in dissociated C. elegans embryonic cells across the cell cycle. Centrosomes were visualized in cells isolated from early-stage embryos (strain JWW69 expressing GFP::SPD-5 and H2B::mCherry). Cells were plunge-frozen, thinned by cryo-FIB milling, and imaged on a 300 kV Titan Krios with a K2 Summit detector, Volta phase plate, and energy filter. 12 tomograms were collected at centrosomes in interphase, prophase, metaphase, anaphase, and telophase stages. Comprehensive annotations of centrioles, microtubules, ribosomes, membranes, and pericentriolar material are provided. Tilt series were aligned using patch tracking in IMOD, and tomograms were reconstructed using Warp.