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Dataset

CryoET of near-minimal cells Mycoplasma mycoides JCVI-Syn3A for the development of subtomogram averaging pipelines

  • Dataset ID:DS-10442

Release Date: 2024-10-15

Last Modified: 2024-10-15

key visualization for CryoET of near-minimal cells Mycoplasma mycoides JCVI-Syn3A for the development of subtomogram averaging pipelines

Dataset Overview

Raw movie stacks, tilt series, alignments and cryo-eletron tomograms of synthetically reduced genome cells. JCVI-Syn3A has 493 genes (about half of the genes of the WT Mycoplasma Mycoides Capri GM12). Samples were prepared by plunge-freezing via Leica GP2. Contained data was collected on a Krios G4 using a Falcon 4i detector and SelctrisX energy filter. Tomograms were reconstructed using AreTomo3 v1.0.23. The deposition also includes locations of cytosolic ribosomes identified using slabpick and membrane segmenations created using membrain-seg.

Publications

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Related Databases

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Runs

262 of 262 Runs

Run Name

Tilt Series Quality Score

Annotated Objects

key visualization for 24aug01b_Position_1
24aug01b_Position_1

Run ID: RN-16201

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_10
24aug01b_Position_10

Run ID: RN-16202

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_10_2
24aug01b_Position_10_2

Run ID: RN-16203

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_10_4
24aug01b_Position_10_4

Run ID: RN-16204

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_11_4
24aug01b_Position_11_4

Run ID: RN-16205

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_12
24aug01b_Position_12

Run ID: RN-16206

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_12_3
24aug01b_Position_12_3

Run ID: RN-16207

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_14_3
24aug01b_Position_14_3

Run ID: RN-16208

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_15_3
24aug01b_Position_15_3

Run ID: RN-16209

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_15_5
24aug01b_Position_15_5

Run ID: RN-16210

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_16_2
24aug01b_Position_16_2

Run ID: RN-16211

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_17
24aug01b_Position_17

Run ID: RN-16212

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_1_2
24aug01b_Position_1_2

Run ID: RN-16213

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_1_4
24aug01b_Position_1_4

Run ID: RN-16214

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_1_5
24aug01b_Position_1_5

Run ID: RN-16215

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_20
24aug01b_Position_20

Run ID: RN-16216

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_20_2
24aug01b_Position_20_2

Run ID: RN-16217

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_20_3
24aug01b_Position_20_3

Run ID: RN-16218

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_21
24aug01b_Position_21

Run ID: RN-16219

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_21_2
24aug01b_Position_21_2

Run ID: RN-16220

3 - Moderate
  • cytosolic ribosome
  • membrane
  • 1
  • 2
  • 3
  • 4
  • 5
  • 14