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Dataset

CryoET of near-minimal cells Mycoplasma mycoides JCVI-Syn3A for the development of subtomogram averaging pipelines

  • Dataset ID:DS-10442

Release Date: 2024-10-15

Last Modified: 2024-10-15

key visualization for CryoET of near-minimal cells Mycoplasma mycoides JCVI-Syn3A for the development of subtomogram averaging pipelines

Dataset Overview

Raw movie stacks, tilt series, alignments and cryo-eletron tomograms of synthetically reduced genome cells. JCVI-Syn3A has 493 genes (about half of the genes of the WT Mycoplasma Mycoides Capri GM12). Samples were prepared by plunge-freezing via Leica GP2. Contained data was collected on a Krios G4 using a Falcon 4i detector and SelctrisX energy filter. Tomograms were reconstructed using AreTomo3 v1.0.23. The deposition also includes locations of cytosolic ribosomes identified using slabpick and membrane segmenations created using membrain-seg.

Publications

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Related Databases

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Runs

262 of 262 Runs

Run Name

Tilt Series Quality Score

Annotated Objects

key visualization for 24aug01b_Position_53_2
24aug01b_Position_53_2

Run ID: RN-16269

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_53_3
24aug01b_Position_53_3

Run ID: RN-16270

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_53_6
24aug01b_Position_53_6

Run ID: RN-16271

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24aug01b_Position_54_3
24aug01b_Position_54_3

Run ID: RN-16272

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_25
24jul16a_Position_25

Run ID: RN-16309

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_26_3
24jul16a_Position_26_3

Run ID: RN-16310

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_27
24jul16a_Position_27

Run ID: RN-16311

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_28
24jul16a_Position_28

Run ID: RN-16312

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_30
24jul16a_Position_30

Run ID: RN-16313

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_30_2
24jul16a_Position_30_2

Run ID: RN-16314

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_32
24jul16a_Position_32

Run ID: RN-16315

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_33
24jul16a_Position_33

Run ID: RN-16316

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_35_2
24jul16a_Position_35_2

Run ID: RN-16317

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_35_3
24jul16a_Position_35_3

Run ID: RN-16318

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_5
24jul16a_Position_5

Run ID: RN-16319

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_5_2
24jul16a_Position_5_2

Run ID: RN-16320

3 - Moderate
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_5_3
24jul16a_Position_5_3

Run ID: RN-16321

4 - Good
  • cytosolic ribosome
  • membrane
key visualization for 24jul16a_Position_5_4
24jul16a_Position_5_4

Run ID: RN-16322

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul24a_Position_25_4
24jul24a_Position_25_4

Run ID: RN-16419

5 - Excellent
  • cytosolic ribosome
  • membrane
key visualization for 24jul24a_Position_49_4
24jul24a_Position_49_4

Run ID: RN-16453

5 - Excellent
  • cytosolic ribosome
  • membrane
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