Dataset
Plasma membranes isolated by unroofing - Grid 08
- Dataset ID:DS-10433
Release Date: 2025-01-08
Last Modified: 2025-01-08
Dataset Overview
Michalak, Sun, Sochacki et al. 2024 unroofing manuscript contains 10 grids. This is grid 8 of 10 and contains apical membranes of HEK293 cells. The cells are expressing FerriTag and GFP-FKBP-LCa (clathrin light chain) off of plasmids. The cells were transfected and grown on coverslips coated with fibronectin overnight, then adhered to Quantifoil Au 300 R1.2/1.3 grids coated with poly-lysine prior to removing the basal membrane and cytoplasm with 0.7 bar of paraformaldehyde. Unroofing occurred immediately prior to vitrification.
Authors
Willy W. Sun, Dennis J Michalak, Kem A. Sochacki, Jenny E. Hinshaw, Justin W. Taraska
Publications
Related Databases
EMDB ID:EMD-44921
Runs
20 of 20 Runs
Run Name | Tilt Series Quality Score | Annotated Objects | ||
|---|---|---|---|---|
 | ASB2_ts1 Run ID: RN-16964 | 5 - Excellent | -- | |
 | ASB2_ts10 Run ID: RN-16965 | 5 - Excellent | -- | |
 | ASB2_ts14 Run ID: RN-16966 | 5 - Excellent | -- | |
 | ASB2_ts15 Run ID: RN-16967 | 5 - Excellent | -- | |
 | ASB2_ts16 Run ID: RN-16968 | 5 - Excellent | -- | |
 | ASB2_ts18 Run ID: RN-16969 | 5 - Excellent | -- | |
 | ASB2_ts19 Run ID: RN-16971 | 5 - Excellent | -- | |
 | ASB2_ts2 Run ID: RN-16974 | 5 - Excellent | -- | |
 | ASB2_ts20 Run ID: RN-16975 | 5 - Excellent | -- | |
 | ASB2_ts21 Run ID: RN-16977 | 5 - Excellent | -- | |
 | ASB2_ts22 Run ID: RN-16979 | 5 - Excellent | -- | |
 | ASB2_ts23 Run ID: RN-16981 | 5 - Excellent | -- | |
 | ASB2_ts25 Run ID: RN-16983 | 5 - Excellent | -- | |
 | ASB2_ts27 Run ID: RN-16985 | 5 - Excellent | -- | |
 | ASB2_ts3 Run ID: RN-16987 | 5 - Excellent | -- | |
 | ASB2_ts4 Run ID: RN-16989 | 5 - Excellent | -- | |
 | ASB2_ts5 Run ID: RN-16991 | 5 - Excellent | -- | |
 | ASB2_ts7 Run ID: RN-16993 | 5 - Excellent | -- | |
 | ASB2_ts8 Run ID: RN-16994 | 5 - Excellent | -- | |
 | ASB2_ts9 Run ID: RN-16996 | 5 - Excellent | -- |