Dataset
Plasma membranes isolated by unroofing - Grid 07
- Dataset ID:DS-10432
Release Date: 2025-01-08
Last Modified: 2025-01-08
Photo Caption: Central slab from a representative tomogram of a Homo sapiens sample.
Dataset Overview
Michalak, Sun, Sochacki et al. 2024 unroofing manuscript contains 10 grids. This is grid 7 of 10 and contains apical membranes of HEK293 cells. The cells are expressing FerriTag and GFP-FKBP-LCa (clathrin light chain) off of plasmids. The cells were transfected and grown on coverslips coated with fibronectin overnight, then adhered to Quantifoil Au 300 R1.2/1.3 grids coated with poly-lysine prior to removing the basal membrane and cytoplasm with 0.7 bar of paraformaldehyde. Unroofing occurred immediately prior to vitrification.
Authors
- Willy W. Sun,
- Dennis J Michalak,
- Kem A. Sochacki,
- Jenny E. Hinshaw,
- Justin W. Taraska
Publications
Related Databases
EMDB ID:EMD-44921
Runs
71 of 71 Runs
Run Name | Tilt Series Quality Score | Objects | ||
|---|---|---|---|---|
 | ASB1tomo003 Run ID: RN-17019 | 5 - Excellent | -- | |
 | ASB1tomo005 Run ID: RN-17021 | 5 - Excellent | -- | |
 | ASB1tomo006 Run ID: RN-17023 | 5 - Excellent | -- | |
 | ASB1tomo009 Run ID: RN-17026 | 5 - Excellent | -- | |
 | ASB1tomo010 Run ID: RN-17028 | 5 - Excellent | -- | |
 | ASB1tomo011 Run ID: RN-17031 | 5 - Excellent | -- | |
 | ASB1tomo012 Run ID: RN-17035 | 5 - Excellent | -- | |
 | ASB1tomo013 Run ID: RN-17038 | 5 - Excellent | -- | |
 | ASB1tomo014 Run ID: RN-17042 | 5 - Excellent | -- | |
 | ASB1tomo016 Run ID: RN-17045 | 5 - Excellent | -- | |
 | ASB1tomo017 Run ID: RN-17049 | 5 - Excellent | -- | |
 | ASB1tomo021 Run ID: RN-17054 | 5 - Excellent | -- | |
 | ASB1tomo1158 Run ID: RN-17058 | 5 - Excellent | -- | |
 | ASB1tomo1159 Run ID: RN-17062 | 5 - Excellent | -- | |
 | ASB1tomo1162 Run ID: RN-17067 | 5 - Excellent | -- | |
 | ASB1tomo1163 Run ID: RN-17072 | 5 - Excellent | -- | |
 | ASB1tomo1166 Run ID: RN-17077 | 5 - Excellent | -- | |
 | ASB1tomo1167 Run ID: RN-17082 | 5 - Excellent | -- | |
 | ASB1tomo1168 Run ID: RN-17087 | 5 - Excellent | -- | |
 | ASB1tomo1169 Run ID: RN-17092 | 5 - Excellent | -- |
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