Deposition

Cryo-ET of isolated segments of synaptic terminals, synaptosomes, from rat hippocampi

Deposition ID:CZCDP-10313

Release Date: 2024-11-26

Last Modified: 2024-11-26

key visualization for Cryo-ET of isolated segments of synaptic terminals, synaptosomes, from rat hippocampi

Photo Caption: Central slab of 4 representative tomograms with selected sets of annotations.

Deposition Overview

CryoET tomograms of synaptosomes for the development of segmentation and subtomogram averaging pipelines for synaptic vesicle proteins, post-synaptic receptors, etc. Each dataset has a slightly different sample preparation conditions. The tomograms collectively have a combination of full synaptosomes, presynaptic terminals, and other co-purified organelles that are not synaptosomes such as mitochondria.

Authors

Deposition Data

Annotations:484

Publications

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Related Databases

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Methods Summary

Annotations	Method Type	Method Details	Method Links
484	Automated	Prediction using membrain-seg without rescaling and weights MemBrain_seg_v10_alpha.ckpt	Source Code:membrain-seg +1 more

Deposited Data

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Data Contents

Tilt SeriesAvailable

FramesAvailable

CTFAvailable

AlignmentAvailable

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484 of 484 Annotations

Annotation Name	Object Shape Type	Method Type	Deposited In
100membrane Annotation ID: AN-69243	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_7
100membrane Annotation ID: AN-69242	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_6_2
100membrane Annotation ID: AN-69241	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_6
100membrane Annotation ID: AN-69240	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_5_2
100membrane Annotation ID: AN-69239	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_4_2
100membrane Annotation ID: AN-69238	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_4
100membrane Annotation ID: AN-69237	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_36
100membrane Annotation ID: AN-69236	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_35
100membrane Annotation ID: AN-69235	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_31
100membrane Annotation ID: AN-69234	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_30_3
100membrane Annotation ID: AN-69233	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_30_2
100membrane Annotation ID: AN-69232	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_2_2
100membrane Annotation ID: AN-69231	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_27_2
100membrane Annotation ID: AN-69230	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_27
100membrane Annotation ID: AN-69229	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_22_3
100membrane Annotation ID: AN-69228	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_22_2
100membrane Annotation ID: AN-69227	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_21_2
100membrane Annotation ID: AN-69226	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_21
100membrane Annotation ID: AN-69225	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_1_2
100membrane Annotation ID: AN-69224	SegmentationMask	Automated	Dataset: Synaptosomes from rat hippocampi (180 mg, 800 rpm, Au grid, high tilt range, 1:50)Run: 24oct15b_Position_16_2