Dataset
Large-scale cryo-ET dataset of affinity-captured organelles (TMEM192 + Bafilomycin + mLST8 SpyCatcher-Gold) HEK293T cells
- Dataset ID:DS-10463
Release Date: 2025-12-18
Last Modified: 2025-12-18
Photo Caption: Central slab from a representative tomogram of a Homo sapiens sample with selected set of annotations.
Dataset Overview
This large-scale cryo-electron tomography (cryo-ET) dataset contains tilt series from affinity-captured organelles derived from HEK293T lysates. The experimental design utilizes electron microscopy grids functionalized with anti-GFP nanobodies to capture organelles tagged with GFP-TMEM192 from cells treated with Bafilomycin A1 for 3 hours. The mTORC1 component mLST8 was targeted using a SpyCatcher system conjugated to 3 nm gold nanoparticles. The complete dataset includes raw frames, alignments, tomograms, and annotations. All data were processed using the automated AreTomo3 pipeline, with semantic segmentations generated by both SABER and Membrain-Seg.
Authors
Publications
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Related Databases
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Runs
1,361 of 1,361 Runs
Run Name | Tilt Series Quality Score | Objects | ||
|---|---|---|---|---|
 | 25jan16a_Position_1 Run ID: RN-21223 | 3 - Moderate |
| |
 | 25jan16a_Position_100 Run ID: RN-21224 | 4 - Good |
| |
 | 25jan16a_Position_100_2 Run ID: RN-21225 | 3 - Moderate |
| |
 | 25jan16a_Position_100_3 Run ID: RN-21226 | 4 - Good |
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 | 25jan16a_Position_100_4 Run ID: RN-21227 | 4 - Good |
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 | 25jan16a_Position_100_5 Run ID: RN-21228 | 4 - Good |
| |
 | 25jan16a_Position_101 Run ID: RN-21229 | 3 - Moderate |
| |
 | 25jan16a_Position_101_2 Run ID: RN-21230 | 3 - Moderate |
| |
 | 25jan16a_Position_101_3 Run ID: RN-21231 | 5 - Excellent |
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 | 25jan16a_Position_101_4 Run ID: RN-21232 | 4 - Good |
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 | 25jan16a_Position_101_5 Run ID: RN-21233 | 3 - Moderate |
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 | 25jan16a_Position_102 Run ID: RN-21234 | 3 - Moderate |
| |
 | 25jan16a_Position_102_2 Run ID: RN-21235 | 4 - Good |
| |
 | 25jan16a_Position_102_3 Run ID: RN-21236 | 3 - Moderate |
| |
 | 25jan16a_Position_102_4 Run ID: RN-21237 | 4 - Good |
| |
 | 25jan16a_Position_102_5 Run ID: RN-21238 | 4 - Good |
| |
 | 25jan16a_Position_102_6 Run ID: RN-21239 | 3 - Moderate |
| |
 | 25jan16a_Position_103 Run ID: RN-21240 | 3 - Moderate |
| |
 | 25jan16a_Position_103_2 Run ID: RN-21241 | 4 - Good |
| |
 | 25jan16a_Position_103_3 Run ID: RN-21242 | 3 - Moderate |
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