Dataset
Large-scale cryo-ET dataset of affinity-captured organelles (TMEM192 + Bafilomycin + V1A SpyCatcher-Gold) HEK293T cells
- Dataset ID:DS-10462
Release Date: 2025-12-18
Last Modified: 2025-12-18
Photo Caption: Central slab from a representative tomogram of a Homo sapiens sample with selected set of annotations.
Dataset Overview
This large-scale cryo-electron tomography (cryo-ET) dataset contains tilt series from affinity-captured organelles derived from HEK293T lysates. The experimental design utilizes electron microscopy grids functionalized with anti-GFP nanobodies to capture organelles tagged with GFP-TMEM192 from cells treated with Bafilomycin A1 for 3 hours. The V-ATPase V1A subunit was targeted using a SpyCatcher system conjugated to 3 nm gold nanoparticles. The complete dataset includes raw frames, alignments, tomograms, and annotations. All data were processed using the automated AreTomo3 pipeline, with semantic segmentations generated by both SABER and Membrain-Seg.
Authors
Publications
Not Submitted
Related Databases
Not Submitted
Runs
2,133 of 2,133 Runs
Run Name | Tilt Series Quality Score | Objects | ||
|---|---|---|---|---|
 | 24oct03c_Position_10 Run ID: RN-21317 | 4 - Good |
| |
 | 24oct03c_Position_101 Run ID: RN-21320 | 5 - Excellent |
| |
 | 24oct03c_Position_101_2 Run ID: RN-21324 | 4 - Good |
| |
 | 24oct03c_Position_101_3 Run ID: RN-21327 | 4 - Good |
| |
 | 24oct03c_Position_101_4 Run ID: RN-21330 | 5 - Excellent |
| |
 | 24oct03c_Position_101_6 Run ID: RN-21333 | 5 - Excellent |
| |
 | 24oct03c_Position_101_7 Run ID: RN-21336 | 3 - Moderate |
| |
 | 24oct03c_Position_101_8 Run ID: RN-21339 | 4 - Good |
| |
 | 24oct03c_Position_101_9 Run ID: RN-21342 | 4 - Good |
| |
 | 24oct03c_Position_10_2 Run ID: RN-21481 | 4 - Good |
| |
 | 24oct03c_Position_102_2 Run ID: RN-21345 | 4 - Good | -- | |
 | 24oct03c_Position_102_3 Run ID: RN-21347 | 5 - Excellent |
| |
 | 24oct03c_Position_102_5 Run ID: RN-21350 | 4 - Good |
| |
 | 24oct03c_Position_102_6 Run ID: RN-21353 | 4 - Good |
| |
 | 24oct03c_Position_102_7 Run ID: RN-21356 | 4 - Good | -- | |
 | 24oct03c_Position_103_6 Run ID: RN-21359 | 5 - Excellent |
| |
 | 24oct03c_Position_103_7 Run ID: RN-21362 | 5 - Excellent |
| |
 | 24oct03c_Position_103_8 Run ID: RN-21364 | 4 - Good |
| |
 | 24oct03c_Position_103_9 Run ID: RN-21368 | 4 - Good | -- | |
 | 24oct03c_Position_104 Run ID: RN-21370 | 4 - Good |
|
- 1
- 2
- 3
- 4
- 5
- 107