Dataset
Large-scale cryo-ET dataset of affinity-captured organelles (LAMP1 + untreated) HEK293T cells
- Dataset ID:DS-10457
Release Date: 2025-12-18
Last Modified: 2025-12-18
Photo Caption: Central slab from a representative tomogram of a Homo sapiens sample with selected set of annotations.
Dataset Overview
This large-scale cryo-electron tomography (cryo-ET) dataset contains tilt series from affinity-captured organelles derived from HEK293T lysates. The experimental design utilizes electron microscopy grids functionalized with anti-GFP nanobodies to capture organelles tagged with GFP-LAMP1 from untreated cells. The complete dataset includes raw frames, alignments, tomograms, and annotations. All data were processed using the automated AreTomo3 pipeline, with semantic segmentations generated by both SABER and Membrain-Seg.
Authors
Publications
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Related Databases
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Runs
941 of 941 Runs
Run Name | Tilt Series Quality Score | Objects | ||
|---|---|---|---|---|
 | 24apr03a_Position_1 Run ID: RN-20282 | 5 - Excellent |
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 | 24apr03a_Position_10 Run ID: RN-20283 | 5 - Excellent |
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 | 24apr03a_Position_100_10 Run ID: RN-20284 | 4 - Good |
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 | 24apr03a_Position_100_12 Run ID: RN-20285 | 5 - Excellent |
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 | 24apr03a_Position_100_14 Run ID: RN-20286 | 4 - Good |
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 | 24apr03a_Position_100_15 Run ID: RN-20287 | 4 - Good |
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 | 24apr03a_Position_100_2 Run ID: RN-20288 | 4 - Good | -- | |
 | 24apr03a_Position_100_3 Run ID: RN-20289 | 3 - Moderate |
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 | 24apr03a_Position_100_4 Run ID: RN-20290 | 4 - Good |
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 | 24apr03a_Position_100_7 Run ID: RN-20291 | 4 - Good |
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 | 24apr03a_Position_100_8 Run ID: RN-20292 | 3 - Moderate |
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 | 24apr03a_Position_100_9 Run ID: RN-20293 | 4 - Good |
| |
 | 24apr03a_Position_101_13 Run ID: RN-20294 | 3 - Moderate |
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 | 24apr03a_Position_101_14 Run ID: RN-20295 | 3 - Moderate |
| |
 | 24apr03a_Position_101_2 Run ID: RN-20296 | 4 - Good |
| |
 | 24apr03a_Position_101_4 Run ID: RN-20297 | 5 - Excellent |
| |
 | 24apr03a_Position_101_6 Run ID: RN-20298 | 3 - Moderate |
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 | 24apr03a_Position_102 Run ID: RN-20299 | 4 - Good | -- | |
 | 24apr03a_Position_10_2 Run ID: RN-20359 | 4 - Good |
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 | 24apr03a_Position_102_14 Run ID: RN-20300 | 4 - Good |
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