CryoET of NPC1-Deficient Human HEK293T Cells with Annotations for Multiple Cellular Features

Human HEK293T cells lacking the lysosomal cholesterol transporter NPC1 were cultured and plunge-frozen. This dataset contains tomograms with comprehensive annotations of cellular features including lysosome clusters within larger membrane compartments, abnormal mitochondrial morphology, microtubules, and ribosomes throughout the cytoplasm. Membranes are annotated to reveal the complex architecture of lysosomal compartments and their spatial relationships with other organelles.